Eppendorf Micromanipulator 5171 Manual
Download ->>->>->> https://urlgoal.com/2ty50U
How to Use the Eppendorf Micromanipulator 5171 for Microinjection
The Eppendorf Micromanipulator 5171 is a device that allows you to perform precise and efficient microinjection of cells. It combines the features of a mechanical system with the advantages of an electromotive system, giving you direct control over the movement of the microcapillary. In this article, we will explain how to use the Eppendorf Micromanipulator 5171 for microinjection, and what are its benefits and applications.
What is the Eppendorf Micromanipulator 5171
The Eppendorf Micromanipulator 5171 is a device that consists of three components[^1^]:
A module unit that attaches to the microscope stage and holds the microcapillary.
A control board that allows you to adjust the speed, angle, limit, and axial mode of the microcapillary.
A power unit that supplies power to the module unit and the control board.
The Eppendorf Micromanipulator 5171 can be connected to the Eppendorf Transjector 5246, which enables semi-automatic injection by activating the injection movement and pressure simultaneously at the push of a button[^1^].
How to Use the Eppendorf Micromanipulator 5171 for Microinjection
To use the Eppendorf Micromanipulator 5171 for microinjection, you need to follow these steps:
Attach the module unit to the microscope stage using the appropriate adapter. You can mount it on either the left or the right side of the stage[^1^].
Connect the power unit to a power outlet and turn it on. Connect the module unit and the control board to the power unit using the interface cables[^1^]. If you are using the Transjector 5246, connect it to the control board as well[^2^].
Fix the microcapillary onto the module unit at any angle and direction using the capillary holder. Make sure that the tip of the capillary is visible under the microscope[^1^].
Adjust the speed mode of the microcapillary by pressing the SPEED key on the control board. You can choose between slow and fast speed modes, indicated by a light-emitting diode. The slow speed mode is suitable for fine movements, while the fast speed mode is suitable for coarse movements[^1^].
Adjust the angle of the microcapillary by pressing and holding the AXIAL key on the control board. You can rotate the capillary in any direction using the cursor keys. The angle mode is indicated by a light-emitting diode[^1^].
Set or switch off the Z limit by pressing and holding the LIMIT key on
the control board. The Z limit is a safety feature that prevents you from moving
the capillary beyond a certain depth. You can adjust
the Z limit using
the cursor keys. The Z limit mode is indicated by a light-emitting diode[^1^].
Switch on or off
the axial mode by pressing
the AXIAL key on
the control board. The axial mode allows you to insert
and remove
the capillary axially into
and out of
the cell, which reduces
the damage to
the cell membrane. The axial mode is indicated by a light-emitting diode[^1^].
Exit or enter
the work area by pressing
the HOME/CLEAN key on
the control board. The work area is where you perform
the microinjection. The HOME/CLEAN key allows you to move
the capillary away from
the work area for cleaning or changing
the capillary, and then return
to
the same position[^1^].
Perform
the microinjection by pressing
the joystick key on
the control board or
the Transjector 5246 button. The joystick key allows you to move
the capillary in any direction using
the joystick. The Transjector 5246 button allows 061ffe29dd